ESPR刊登用3D细胞培养通过PCRARRAY分析化合物毒性

沃吉基因（PCR Array）好文分享之IF:5.19

文章摘要

此前，我们发现作为3D球体培养的鸡LMH细胞系可能是鸡胚胎肝细胞(CEH)的体外化学筛选。在本研究中，测定了暴露于双酚A（BPA）、ffeBPA替代化合物（BPF、TGSH、DD-70、BPAF，BPSIP）和17β雌二醇（E2）后，LMH 3D球状体的细胞毒性和mRNA表达。

文章方法

Common name, CASRN, structure, and logP of bisphenol A (BPA) and five BPA replacement compounds and their corresponding LC50 values (μM)±SEM in LMH 3D spheroids.

¹LogP values from ACD lab on Chemspider.

Gene expression was determined using three customized chicken PCR arrays: 1) ToxChip, 2) AestroChip, 3) ComboChip.

Principal component analysis of signifcant genes on the ToxChip and AestroChip arrays in A) LMH 3D spheroids and B) CEH following exposure to BPA, BPA replacement compounds (BPF, TGSH, DD-70, BPAF, and BPSIP), and 17β estradiol(E2).

文章来源

Sharin T, Crump D, O’Brien J M. Toxicity screening of bisphenol A replacement compounds: cytotoxicity and mRNA expression in LMH 3D spheroids[J]. Environmental Science and Pollution Research, 2022: 1-10.

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