Nature Neurosci—睡眠研究重磅突破：压后皮层调控REM睡眠

中文摘要

快速眼动（REM）睡眠是一种以骨骼肌麻痹和大脑皮层激活为特征的睡眠状态。然而，全皮层动力学及其在调节REM睡眠中的作用仍不清楚。在这里，科学家发现，在小鼠中，REM睡眠伴随着高度模式化的皮层活动波，其中压后皮层（RSC）是主要的起始点。RSC 2/3层锥体神经元的双光子成像揭示了REM睡眠期间两种不同的群体活动模式。这些活动编码了两个连续的REM睡眠亚阶段，其特征是面部运动和自主活动的对比以及可区分的脑电图θ振荡。在REM睡眠期间RSC的闭环光遗传学失活通过抑制REM亚阶段转变改变了皮层活动动力学并缩短了REM睡眠时间。这些结果突出了RSC在控制皮层动力学和调节REM睡眠进展方面的重要作用。

英文摘要

Rapid eye movement (REM) sleep is a sleep state characterized by skeletal muscle paralysis and cerebral cortical activation. Yet, global cortical dynamics and their role in regulating REM sleep remain unclear. Here we show that in mice, REM sleep is accompanied by highly patterned cortical activity waves, with the retrosplenial cortex (RSC) as a major initiation site. Two-photon imaging of layer 2/3 pyramidal neurons of the RSC revealed two distinct patterns of population activities during REM sleep. These activities encoded two sequential REM sleep substages, characterized by contrasting facial movement and autonomic activity and by distinguishable electroencephalogram theta oscillations. Closed-loop optogenetic inactivation of RSC during REM sleep altered cortical activity dynamics and shortened REM sleep duration via inhibition of the REM substage transition. These results highlight an important role for the RSC in dictating cortical dynamics and regulating REM sleep progression.

参考文献：Cortical regulation of two-stage rapid eye movement sleep. Nat Neurosci. 2022 Nov 17. doi: 10.1038/s41593-022-01195-2. Online ahead of print.

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