方法：将小鼠随机分为五组(每组n=5)：AM(成年小鼠)假手术组、AM(成年小鼠)手术组、EM(老年小鼠)假手术组、EM(老年小鼠)手术组和EM-P(伴有PNU的老年小鼠)手术组。术后24h评定认知水平。采用酶联免疫吸附试验(ELISA)和定量聚合酶链反应(qPCR)检测外周血和脾脏单核细胞的促炎细胞因子(TNF-α、IL-6和IL-10)。免疫荧光法检测海马M2巨噬细胞水平。流式细胞仪检测脾脏CD11b/c+α7 nAChR+ 细胞。
结果：术后24小时，老年小鼠POCD较成年小鼠显著升高。老年手术组小鼠(EM)的促炎因子TNF-α和IL-6高于成年手术组小鼠(AM)和老年-P手术组小鼠(EM-P)；EM手术组小鼠的抗炎因子IL-10和M2巨噬细胞低于AM手术组小鼠和EM-P手术组小鼠。EM手术组小鼠脾细胞CD11b/c+α7 nAChR+ 细胞数量减少。
文献来源：Gong M, Wang G, Li G, et al. Dysfunction of Inflflammation-Resolving Pathways Is Associated with Postoperative Cognitive Decline in Elderly Mice.Behav Brain Res 2020 May 27;386. DOI：10.1016/j.bbr.2020.112538.
Dysfunction of Inflammation-Resolving Pathways Is Associated with Postoperative Cognitive Decline in Elderly Mice
Background: Postoperative cognitive dysfunction (POCD) refers to a reversible, perioperative mental disorder. POCD increases the likelihood of postoperative complications and the risk for postoperative mortality, typically among elderly patients (age 65 or older). The importance of the cholinergic anti-inflammatory pathway (CAP) in resolving neuro-inflammatory and cognitive decline caused by sterile trauma has been recognized. We speculate that the POCD in elderly mice is associated with dysfunction of CAP.
Methods: Mice were assigned to several groups (n = 5 in each group): AM (adult mice) Sham, AM (adult mice) Surgery, EM (elderly mice) Sham, EM (elderly mice) Surgery, and EM-P (elderly mice with PNU) Surgery. At 24h after surgery, assessed the cognitive levels. Pro-inflammatory cytokines in peripheral blood and splenic monocytes (TNF-α, IL-6 and IL-10) were assessed by ELISA and qPCR. Levels of M2 macrophages in hippocampus were visualized by immunofluorescence. Detecting CD11b/c+α7 nAChR+ cells in the spleens with flow cytometry.
Results: At postoperative 24 hours, elderly mice exhibited significantly increased POCD compared with adult mice. The proinflammatory factor TNF-α and IL-6 were higher among elderly surgery mice (EM) compared with adult surgery (AM) and elderly-P surgery mice (EM-P); the anti-inflammatory factor IL-10 and M2 macrophages were lower among EM surgery mice compared with AM surgery and EM-P surgery mice. The CD11b/c+α7 nAChR+ population of splenocytes was reduced in the EM surgery mice.
Conclusions: The exaggerated and persistent cognitive decline and inflammatory response among elderly mice were associated with dysfunction of CAP, and these phenomena were reversed by α7nAch receptor agonists.