与丙泊酚相比七氟烷能保护线粒体功能并减轻心肌缺血/再灌注损伤

2020
08/26

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背景:减轻心肌缺血再灌注(I/R)损伤的药物干预包括麻醉药的使用。七氟烷和丙泊酚已被证明可通过不同的分子机制发挥心脏保护作用。我们的研究假设七氟烷不同于丙泊酚麻醉,可通过线粒体疾病机制预防I/R损伤而产生心脏保护作用。

方法:将雄性新西兰大白兔(n=42)冠状动脉夹闭30min,再灌注3h。戊巴比妥诱导后,大白兔接受七氟烷或丙泊酚维持全身麻醉。TTC染色后用重量法测定梗死面积。分离心肌线粒体,用Clark电极测定线粒体耗氧量。线粒体呼吸链复合物活性(I-IV)采用特异性分析方法进行测定。实验数据以均数±标准差表示。

结果:与丙泊酚麻醉相比,七氟烷麻醉可显著减少心肌梗死面积(与丙泊酚比较p=0.0275)。丙泊酚麻醉组的白兔线粒体呼吸控制率明显降低(与假手术组相比p=0.01909),呼吸复合物I(与假手术组相比p=0.0147 ;与七氟烷组相比p<0.01)和呼吸复合物IV(与假手术组相比p=0.0181)的活性受损。七氟烷麻醉组白兔未出现线粒体功能障碍。此外,吸入七氟烷麻醉的白兔在I/R损伤过程中,非活性状态的呼吸复合物I的比例明显较高(与丙泊酚相比p=0.0123)

结论:与丙泊酚麻醉相比,七氟烷麻醉保留了线粒体呼吸并对I/R损伤产生心脏保护作用。

原始文献:Lotz C,  Stumpner J,  Smul TM. Sevoflurane as opposed to propofol anesthesia preserves mitochondrial function and alleviates myocardial ischemia/reperfusion injury.[J].Biomed. Pharmacother. 2020,20:129.

Sevoflurane as opposed to propofol anesthesia preserves mitochondrial function and alleviates myocardial ischemia/reperfusion injury

Abstract

Background: Pharmacological interventions reducing myocardial ischemia and reperfusion (I/R) injury include the administration of anesthetics. Both sevoflurane as well as propofol have been shown to elicit cardiac protection via distinct molecular mechanisms. We investigated the hypothesis that Sevoflurane in contrary to propofol anesthesia elicits cardiac protection against I/R-injury via mitochondrial mechanisms of disease.

Methods: Male New Zealand white rabbits (n=42) were subjected 30 min of coronary artery occlusion followed by 3 h of reperfusion.After induction with pentobarbital, the animals either received Sevoflurane or propofol to maintain general anesthesia. Infarct size was determined gravimetrically after triphenyltetrazolium chlorid-staining. Cardiac mitochondria were isolated and mitochondrial oxygen consumption was measured using a Clark electrode.Mitochondrial respiratory chain complex activities (I-IV) were analyzed utilizing specifific assays. Data are mean ± SD.

Results: Sevoflurane anesthesia significantly decreased the resulting myocardial infarct size compared to propofol anesthesia (p =0.0275 vs. propofol).Mitochondria from animals receiving propofol anesthesia showed a signifificantly reduced mitochondrial respiratory control ratio (p = 0.01909 vs. sham) and impaired activities of respiratory complex I (p = 0.0147 vs. sham; p < 0.010 vs. sevoflurane) as well as respiratory complex IV (p =0.0181 vs. sham). Mitochondrial dysfunction was absent in Sevoflurane anesthesized animals. Furthermore, a significantly higher portion of complex I was found to be in its deactive form during I/R-injury in animals receiving sevoflurane anesthesia (p =0.0123 vs. propofol).

Conclusions: Sevoflurane as opposed to propofol anesthesia preserved mitochondrial respiration and elicited cardiac protection against I/R-injury.

本文由作者自行上传,并且作者对本文图文涉及知识产权负全部责任。如有侵权请及时联系(邮箱:guikequan@hmkx.cn
关键词:
氟烷,丙泊酚,心肌缺血

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