PCR ARRAY应用指南

PCR ARRAY应用

1 PCR ARRAY应用一：分子机制探究信号通路PCR芯片

2 PCR ARRAY应用二：转录组测序后通路PCR芯片验证

3 PCR ARRAY应用三：生物标志物研究之microRNA PCR 芯片

4 PCR ARRAY应用四：MICRORNA 靶基因PCR芯片

5 PCR ARRAY应用五：肿瘤研究PCR 芯片PCR 

6 PCR ARRAY应用六：转录组测序之后差异基因PCR 芯片

7 PCR ARRAY应用七：细胞功能之氧化应激PCR芯片

8 PCR ARRAY应用八：动物模型之信号通路PCR芯片

9 PCR ARRAY应用九：肿瘤lncRNA PCR芯片

10 PCR ARRAY应用十：分子功能之代谢PCR芯片

案例一：ADAR1通过调节RLR依赖性信号通路来促进KSHV裂解激活

文章信息：ADAR1 Facilitates KSHV Lytic Reactivation by Modulating the RLR-Dependent Signaling Pathway

Huirong Zhang, Guoxin Ni, Blossom Damania

doi: 10.1016/j.celrep.2020.107564

发表杂志：Cell Reports（IF=7）

材料：人iSLK.219细胞，TREx BCBL1-RTA细胞，293FT细胞

实验方法：KSHV病毒感染iSLK.219细胞和BCBL1-TREx-RTA细胞，转染siRNA，后用含有84个目的基因的人类干扰素和受体PCR阵列进行qPCR检测

实验目的：KSHV病毒裂解再激活过程中，ADAR1在信号通路中的作用

使用芯片：人类干扰素和受体PCR阵列

案例二：组蛋白甲基转移酶EZH2 / 1的抑制剂的抗病毒效应研究

文章信息：inhibitors of the Histone Methyltransferases EZH2/1 Induce a Potent Antiviral State and Suppress Infection by Diverse Viral Pathogens

Jesse H. Arbuckle, Paul J. Gardina, David N. Gordon, Heather D. Hickman, Jonathan W. Yewdell, Theodore C. Pierson, Timothy G. Myers, Thomas M. Kristie

DOI: 10.1128/mBio.01141-17

发表杂志：MBIO（IF=6）

材料：HFF细胞

实验方法：先用HSV病毒感染HFF细胞，再用EZH2 / 1的抑制剂处理HFF细胞，后用IFN qPCR芯片检测细胞内差异基因，再通过转录组测序分析抗病毒机制

实验目的：确定IFN和IFN受体PCR阵列中差异表达的基因

使用芯片： IFN qPCR芯片

案例三：LCa淋巴结转移的miRNA特征定义：miR-449a靶向Notch基因并抑制细胞迁移和侵袭

文章信息：Definition of miRNA Signatures of Nodal Metastasis in LCa: miR-449a Targets Notch Genes and Suppresses Cell Migration and Invasion

Hiromichi Kawasaki, Takashi Takeuchi, Filippo Ricciardiello, Angela Lombardi, Elia Biganzoli, Marco Fornili, Davide De Bortoli, Massimo Mesolella

doi: 10.1016/j.omtn.2020.04.006

发表杂志：Molecular Therapy-Nucleic Acids（IF=5）

材料：淋巴结转移（N=23）和无淋巴结转移（N=23）的LCa患者及其邻近的正常对照组（N=30）收集的临床LCa组织样本

实验方法：通过含有309个miRNA的qPCR ARRAY对组织样品检测，分析LCa组织中差异的miRNA

实验目的：确定LCa组织中的特异表达的miRNA

使用芯片：人miRNA ARRAY

案例四：miR-21对lncRNA GAS5的负调控

文章信息：Negative regulation of lncRNA GAS5 by miR-21

Z Zhang, Z Zhu, K Watabe, X Zhang, C Bai, M Xu, F Wu & Y-Y Mo

https://doi.org/10.1038/cdd.2013.110

发表杂志：Cell Death & Differentiation （IF=8）

材料：乳腺癌MCF-7细胞和MDA-MB-231细胞，正常细胞MCF-10A

实验方法：通过83个人类疾病相关IncRNA阵列检测miR-21对IncRNA表达的影响。

实验目的：确定miR-21是否可以调节lncRNA表达

使用芯片：人类疾病相关IncRNA阵列

案例五：小细胞肺癌中Bromodomain和Hedgehog通路靶点的研究

文章信息：Bromodomain and Hedgehog Pathway Targets in Small Cell Lung Cancer

Gurmeet Kaur,1 Russell A. Reinhart, Anne Monks, David Evans, Joel Morris, Eric Polley, and Beverly A. Teicher

doi: 10.1016/j.canlet.2015.12.001

发表杂志：Cancer Letters（IF=6）

材料

实验方法：采用96孔自定义PCR array，对24株小细胞肺癌细胞株进行了Hedgehog、Notch、肺癌和stem-like基因的表达检测。

实验目的：筛选MYC靶基因

使用芯片：定制芯片

案例六：RNA-Seq对大鼠耳蜗噪声损伤后补体成分的研究

文章信息：Transcriptome characterization by RNA-Seq reveals the involvement of the complement components in noise-traumatized rat cochleae

M.Patel, Z.Hu, J.Bard, J.Jamison, Q.Cai, B.H.Hu

https://doi.org/10.1016/j.neuroscience.2013.05.038

发表杂志：Neuroscience（IF=3）

材料：SD大鼠

实验方法：噪音损伤SD大鼠耳蜗，取耳蜗组织进行RNA测序和qPCR ARRAY检测

实验目的：通过凋亡PCR芯片验证RNA测序结果

使用芯片：大鼠凋亡芯片

案例七：斑马鱼对丙烯酰胺毒性的氧化应激研究

文章信息：In vitro and in vivo studies of oxidative stress responses against acrylamide toxicity in zebrafish

Yuta Komoike, Masato Matsuoka

https://doi.org/10.1016/j.jhazmat.2018.11.023

发表杂志：Journal of Hazardous Materials （IF=9）

材料：斑马鱼细胞BRF41和斑马鱼幼苗

实验方法：斑马鱼细胞BRF41和斑马鱼幼苗暴露于丙烯酰胺后，通过PCR array分析氧化应激反应相关基因的表达变化

实验目的：丙烯酰胺对斑马鱼应激信号通路的影响

使用芯片：斑马鱼氧化应激芯片

案例八：敌敌畏对鸡脑组织AMPK信号通路影响的研究

文章信息：Investigation of the effects of dichlorvos poisoning on AMPK signaling pathway in chicken brain tissues

Yanyu Xiao, Xibang Zheng, Guyue Li, Changming Zhou, Cong Wu, Zheng Xu, Guoliang Hu, Xiaoquan Guo, Lin Li, Huabin Cao, Vincent Latigo, Ping Liu

https://doi.org/10.1016/j.envpol.2020.114109

发表杂志：Environmental Pollution （IF=6.5）

材料：白羽肉鸡

实验方法：白羽肉鸡作为本实验的研究对象，按敌敌畏剂量分为三组：对照组，低剂量组和高剂量组。对鸡脑组织使用PCR array进行综合分析

实验目的：研究农药对动物AMPK通路相关基因的影响

使用芯片：鸡AMPK信号通路PCR芯片

案例九：MYC驱动的乳腺癌细胞lncRNA谱的调控

文章信息：MYC-driven regulation of long non-coding RNA profiles in breast cancer cells

Pervin Elvan Tokgun , Onur Tokgun , Serap Kurt , Ayse Gaye Tomatir , Hakan Akca

https://doi.org/10.1016/j.gene.2019.143955

发表杂志：GENE（IF=2）

材料：乳腺癌MCF-7细胞和MDA-MB-231细胞

实验方法：通过c-MYC基因敲除或过表达，对细胞进行乳腺癌LncRNA检测

实验目的：筛选受c-MYC基因调节的lncRNA

使用芯片：人肿瘤lncRNA芯片

案例十：双酚类似物对HepG2细胞的代谢调控

文章信息：Metabolomic modulations of HepG2 cells exposed to bisphenol analogues

Yue S, Yu J, Kong Y, Chen H, Mao M, Ji C, Shao S, Zhu J, Gu J, Zhao M.

DOI: 10.1016/j.envint.2019.05.008

发表杂志：Environment International （IF=7）

材料：HepG2细胞

实验方法：HepG2细胞分别用四种BP （BPA， BPAF，BPF和BPS）处理后，使用糖代谢PCR芯片分析细胞基因表达情况

实验目的：筛选糖代谢差异基因

使用芯片：人糖代谢PCR芯片

客户文献节选

肿瘤PCR ARRAY

Bai Y, Wang J, Han J, et al. BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo[J]. Molecular carcinogenesis, 2017, 56(3): 1137-1149.

WNT PCR ARRAY

Zhao Y, Wang J, Xu C, et al. HEG1 indicates poor prognosis and promotes hepatocellular carcinoma invasion, metastasis, and EMT by activating Wnt/β-catenin signaling[J]. Clinical Science, 2019, 133(14): 1645-1662.

炎症因子PCR ARRAY

Ye F, Zeng Q, Dan G, et al. Nitrogen mustard prevents transport of Fra-1 into the nucleus to promote c-Fos-and FosB-dependent IL-8 induction in injured mouse epidermis[J]. Toxicology letters, 2019.

神经受体PCR ARRAY

Gao B Y, Xu D S, Liu P L, et al. Modified constraint-induced movement therapy alters synaptic plasticity of rat contralateral hippocampus following middle cerebral artery occlusion[J]. Neural Regeneration Research, 2020, 15(6): 1045.

Stst3信号通路

Wang H, Yuan M, Wang S, et al. STAT3 Regulates the Type I IFN-Mediated Antiviral Response by Interfering with the Nuclear Entry of STAT1[J]. International Journal of Molecular Sciences, 2019, 20(19): 4870.

DNA损伤PCR ARRAY

Fang L, Li J, Li W, et al. Assessment of Genomic Instability in Medical Workers Exposed to Chronic Low-Dose X-Rays in Northern China[J]. Dose-Response, 2019, 17(4): 1559325819891378.

代谢ARRAY

Yue S, Yu J, Kong Y, et al. Metabolomic modulations of HepG2 cells exposed to bisphenol analogues[J]. Environment international, 2019, 129: 59-67.

自噬PCR ARRAY

Wu J, Kuang L, Chen C, et al. miR-100-5p-abundant exosomes derived from infrapatellar fat pad MSCs protect articular cartilage and ameliorate gait abnormalities via inhibition of mTOR in osteoarthritis[J]. Biomaterials, 2019, 206: 87-100.

肝癌PCR ARRAY

Ma L, Jiang H, Xu X, et al. Tanshinone IIA mediates SMAD7-YAP interaction to inhibit liver cancer growth by inactivating the transforming growth factor beta signaling pathway[J]. Aging (Albany NY), 2019, 11(21): 9719.

自噬PCR ARRAY

Wang N, Yang B, Muhetaer G, et al. XIAOPI formula promotes breast cancer chemosensitivity via inhibiting CXCL1/HMGB1-mediated autophagy[J]. Biomedicine & Pharmacotherapy, 2019, 120: 109519.

凋亡PCR ARRAY

Li L, Sun Y, Zhang N, Qiu X, Wang L, Luo Q. By regulating miR-182-5p/BCL10/CYCS, sufentanil reduces the apoptosis of umbilical cord mesenchymal stem cells caused by ropivacaine. Biosci Trends. 2019;13(1):49‐57. doi:10.5582t.2018.01291

microRNA PCR ARRAY

miR-125a-5p inhibits tumorigenesis in hepatocellular carcinoma

Xin Xu, Yuquan Tao, Yongjie Niu, Zhixian Wang, Congcong Zhang, Yongchun Yu, Lifang Ma. Aging (Albany NY) 2019 Sep 30; 11(18): 7639–7662. 2019 Sep 17. doi: 10.18632/aging.102276

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